We used one unique mask based on the Digimouse atlas shape since, based on previous registration steps, the actual mouse CT image now has the same shape.
Now, if there would be considerable improvement in either of the sets, then this would suggest that the parameters should be chosen based on previous image analysis step.
Based on previous studies, a key step in vascular calcification appears to be differentiation or transformation of vascular smooth muscle cells into an osteoblastic/chondrocytic phenotype [ 8, 15].
Users can easily revise any parameters in a certain step based on previous results, and the refreshing run will not change the results determined by previous steps in the eRNA's pipeline.
GTP- or GDP-bound GTR1 and GTR2 mutants, and other point mutants of GTR2, were generated using primer-based 2-step PCRs, based on previous reports.
This treatment step was based on previous experience [ 12, 13, 16, 18].
In such situations, it is often advantageous to evaluate hypotheses in a step-by-step manner, choosing each test based on previous findings.
Based on previous studies [ 30], the independent variables were entered in the following steps: (i) Step 1: background caregiver's and patient's socio-demographic characteristics; (ii) Step 2: Patient's CAN and VSSS scores; (iii) Step 3: Patient's LQLP subjective QOL, affect and self-esteem scores; (iv) Step 4: LQLP objective QOL indices; (v) Step 5: patient's psychopathology and GAF scores.
