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The cytokines assayed were selected based on previous screening studies using a 22-cytokine assay [ 12].
However, when a sufficiently large amount of experimental data has been accumulated, it is easy to map new mutant genomes into a phylogenetic tree based on previous screening knowledge labeled on the branches.
Eight mouse strains (three to four mice per strain) were used based on previous screening (Rai et al. 2012), representing the following three healing categories: good (MRL/MpJ, LG/J, LGXSM-6); intermediate (LGXSM-5, LGXSM-35); and poor (C57BL/6J, SM/J, LGXSM-33).
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All samples with genetically unexplained FTLD based on previous screens were assessed for C9ORF72 expansions using the repeat-primed polymerase chain reaction as previously published (Renton et al., 2011).
The surfactant and polymer are selected based on previous laboratory screening and evaluation (Han et al. 2013).
Chelerythrine was selected over sanquinarine based on previous NMR screening and docking studies that suggested chelerythrine binds between α4, α5 and α6 of Bcl-xL [37].
Based on previous expression screening, TSC22D4 thus far had been implicated in pituitary development (Fiorenza et al, 2001) and in the adaptation of renal cells to hypertonicity.
Randomization was stratified based on previous insulin regimen at screening: basal-bolus regimen or another insulin regimen.
The interview questions were developed, based on previous research with chlamydia screening co-ordinators and GP surgery staff [ 7, 9, 10] and informed by constructs of the Theory of Planned Behaviour (TPB) [ 11].
Moreover, based on previous reports including E-SCREEN, ER transactivation, binding assays to the two ER subtypes, and effects observed with the ER antagonists ICI 182,780 and tamoxifen (Gutendorf and Westendorf 2001; Scippo et al. 2004; Vivacqua et al. 2003; Wilson et al. 2004), we can conclude that the estrogenic potential of these four compounds is accomplished through the ERs.
These alternative lower volume assumptions were based on previous experience involving a single screening team working on a part time basis since 2004 to implement a screening demonstration project and the EDETCC of visual inspection based screening which was conducted in Xiangyuan County, Shanxi Province.
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