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Two Sarcocheilichthys species were used as outgroups, based on previous molecular phylogenetic [ 17, 25, 29] and morphological [ 18] studies.
How do our estimates of divergence times compare with those based on previous molecular studies of mustelids [ 12, 14, 17, 41]?
and/or Satyrium nepalense D. Don (both in subfamily Orchidoideae, tribe Diseae) were chosen as functional outgroups, a decision based on previous molecular topologies [ 28, 35, 43, 48].
As discussed by Azuma et al. [ 43], whose data set formed the basis of this study, the first factor is not the case in the present study because we did not use calibration dates based on previous molecular studies.
We have demonstrated their potential phylogenetic utility based on a tree of relationships between honeycreeper species used in our RAD analysis that matches expectation based on previous molecular phylogenetic analyses [ 12].
Based on previous molecular clock estimates, lacertids may have entered Africa (and at the same time split from the Palearctic clade) after Eurasia contacted Africa in the Neogene, some 17 19 million years ago (Mya) [ 7].
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The first topology considered is taken from Figure 27.10 of Cracraft et al [ 53], which is a consensus tree based on previous molecular- and morphology-based phylogenetic reconstructions.
The phylogenetic affinities of this taxon to other members of this clade, living and extinct, are uncertain based on previous morphological and molecular studies.
Based on previous findings of molecular recognition between GnT-V and synthetic substrates, we designed and synthesized a p-iodophenyl-derivatized trisaccharide, 2- 4-iodophenyl)ethyl 6-O-[2- 4-iodophenylo-2- 4-iodophenylcopyranosyl)-α-d-mannopyranosyl]-β-d-glucopyranosidethylGMG, 6-O-[2-O- 2-acetamido-2-deoxy-β-d-glucopyranosyl -α-d-mannopyranosyl]-β-d-glucopyranosidein vitro.
First, a set of candidate genes is now available based on previous medical and molecular biology research [ 23- 25].
The hypothesized groupings of Ag1, Ag4, As1, As8 and As9 were based on previous studies that used molecular markers to evaluate the genetic variation [ 17, 18, 20, 23, 52].
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