Based on previous interaction studies of RING and HECT E3 ligases with E2 enzymes [39], [42], [43], we hypothesize that this increase in hydrophobicity could result in an increase in binding affinity between the E3 and E2 proteins that would enhance p53 degradation.
Polymorphisms in the ADRB2 gene (especially Gly16Arg) have been reported to affect not only the long-term response to ADRB2 agonists but also the risk of asthma in relation to active and passive smoking, and we specifically selected ADRB2 in this study based on previous interaction effects (Israel et al. 2004; Wang et al. 2001; Zhang et al. 2007).
While this could be modelled at an individual level based on previous negotiation interactions, in practice it may more likely be determined for the specific context of use (e.g. instructors could decide the relative weighting of their students' claims compared to other sources of data).
In the biological context, it may sometimes be possible to restrict attention to certain types of models on the grounds that they may be based on previous knowledge of interactions or on commonly used assumptions about kinetics of chemical reactions (e.g. with terms of the Michaeles-Menten type).
Finally, based on previous findings reporting interactions between D2 and D4 receptor genes [e.g., [ 110]], we also examined both potential interactive effects (i.e., quantitatively disproportionate effects based on a combination of polymorphisms of both genes) and potential additive effects (i.e., linearly increasing effects based on a combination of polymorphisms of both genes).
The questionnaire was developed based on previous studies, in interaction with clinicians and other researchers, and a pilot study [ 11, 13, 17].
To assess the effect of CMC of fatty acids on Aβ42 aggregation, based on previous reports on Aβ interactions with SDS and other surfactants [30] we chose three specific concentrations of fatty acids: well above (∼3 5 fold), well below (∼3 5 fold) and near the CMC.
In addition, based on previous literature two factor interactions with onsite revascularisation options, teaching status, age and gender were tested.
Based on previous studies we assessed for interactions by race and neighborhood level poverty rate.
