Of the predicted CDSs, 628 (67.7%, PV3056/3), 630 (67.9%, W73) and 629 (67.8%, P787) were functionally assigned based on previous experimental evidence or database similarity and motif matches.
Also, in order to assess the potential role of polysubstance use, based on previous data from this database indicating a more severe clinical picture in clients reporting a higher number of substances [ 22], we included the number of substance types used during the last 30 days before incarceration.
Newer "knowledge-based" software programs, such as the ones used by the Duke researchers, allow doctors to interact with the system – asking questions and getting answers based on previous cancer cases in the database.
Translated protein sequences from Arabidopsis and rice sHsp genes were retrieved from Phytozome database, based on previous analysis reported in the literature [ 8, 65].
Based on previous studies and the Plant MicroRNA Database (http://bioinformatics.cau.edu.cn/PMRD/), miR156, miR171a, miR390b, and miR168a were selected for investigation.
The possible signal cascades network in platelets after binding of SB to integrin α2β1 was predicted based on previous reports and protein-protein interaction database.
Therefore, this information had to be interpolated by the database curator based on previous literature and the enzyme name (see Figure 3).
Based on previous reports of genetic studies and public databases on the FADS gene cluster 9, 11 and the HapMap project (http://www.hapmap.org), eight relevant FADS SNPs were prescreened and four SNPs (FEN1 rs174537G > T, FADS2 rs174575C > G, FADS2 rs2727270C > T, FADS3 1000778C > T) were selected for further analysis (Table S1).
The first set contained loci from the whole dataset while the second set included only loci identified as novel based on previous studies [ 27, 38– 44] and the dbRIP database [ 45].
