Sentence examples for based on preliminary sequence from inspiring English sources
Additional sequence was obtained by primer walking using taxon-specific primers based on preliminary sequence, paired with standard primers, or with other taxon-specific primers.
A region of high polymorphism in each gene (based on preliminary sequencing in the 27 DL) was sequenced in the association panel.
Predictions for p.Gly884Ser (Gly706Ser) and p.Gly1094Ser (Gly916Ser) based on preliminary sequencing were published previously (12).
The lef-8 and E75 primer pairs were designed based on preliminary genome sequencing information of the virus and transcriptomic data available for C. crangon, respectively.
The panel of anaerobic bacteria was chosen based on preliminary 16S rRNA sequencing data from our laboratory showing that these bacteria are frequently present in CF airway samples during pulmonary exacerbations.
This estimate is a little lower than the prediction of 7 8,000 genes in chromosome 3A based on preliminary results from Roche/454 sequences of sorted 3A chromosome arms (our unpublished results).
Therefore, we had to manually assemble all sequences based on preliminary results from ab-initio gene prediction and cross-species gene reconstruction software.
Flanking primers were designed based on preliminary data of the whole genome sequence of F. noatunensis ssp.
Overall, 104 700 bases (95,6%) of the reference sequence was covered at least 30× depth, considered the minimum necessary for reliable sequence variation detection based on preliminary experiments; 309 of the 343 amplicons were fully covered above this threshold.
Based on preliminary exhaustive phylogenetic analyses, 160 representative sequences were chosen for final tree construction (i.e. a selection of bacterial homologues including all characterized PAL and their closest homologues, all archaeal homologues, a selection of plant and fungi homologues, and all homologues for the remaining eukaryotic phyla, see Methods for details.
Eight isolates submitted to CDC from 1989 to 2006 from clinical specimens were initially identified as members of the genus Burkholderia based on preliminary cellular fatty acid analysis and/or 16S rRNA gene sequencing.
