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Two transcriptional modules were found based on predicted terminators.
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BTH_s39 was mapped to the 3′ UTR of a conserved protein-coding gene, BTH_II2030, based on prediction of a transcription terminator sequence immediately following BTH_s39.
The remaining predicted terminators were localised within coding regions or on the antisense strand and therefore, were not further analysed in this work.
Using this criterion, 556 terminators of the 1068 predicted terminators were selected (52%) (Table 1).
The predicted terminators are located downstream of the coding sequences.
Terminators named "wf" represent terminators classified as unstable terminators in the WebGesTer software (Additional files 2, 3 and 4).> The classification criteria defined in Figure 1 were applied to validate the predicted terminators.
We predicted a minimum length 920 nucleotide GFPuv mRNA transcript based on the predicted ompB promoter transcription start site and untranslated mRNA sequence [45] as well as transcription of 70 nucleotides of the ompA terminator to form mRNA hairpin structures and stop transcription [46].
While we cannot rule out at this time that this species is a stable decay intermediate of the upstream ydgA mRNA, based on the predicted strength of the ydgA rho-independent transcription terminator, we hypothesize that candidate 9 is indeed a ncRNA that contains a significant region of antisense sequence to the 3′ terminus of the adjacent uidC mRNA, which is transcribed in the opposite direction.
Based on the predicted values, we selected 0.1% hybrids that had the highest predicted yields (170/167,910 hybrids).
Based on standardised predicted FEV1 values.
The opposite result was observed for RNA isolated from rickettsiae grown in L929 cells where the majority of the RP827 transcripts protected a 187 base portion of the probe indicating termination at the predicted terminator site.
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