Sentence examples for based on predicted sequence from inspiring English sources

Briefly, distance between genomes was computed as the Manhattan distance based on predicted sequence identity for each oligonucleotide and then trees were generated using hierarchical clustering.

Based on predicted sequence information, we performed PCR cloning of MAPK genes by designing gene-specific primers (Additional file 4: Table S4) and amplifying the transcripts of given tissues of G. hirsutum acc.

The phylogenetic tree based on predicted protein sequences of PBF sequences from different grasses agrees with their known phylogenetic relationships (fig. 2 A).

As described elsewhere [ 39] gene models for MPKs and MKKs were targeted for phylogenetic study and a nomenclature based on predicted protein sequence similarity with Arabidopsis and rice was adopted to reflect putative orthology.

Based on predicted amino sequence alignments, an additional five unigenes appear to be truncated by approximately 60 nucleotides (20 amino acids) or less at their N-termini (Table  9).

Based on predicted coding sequences, primers were designed to amplify partial coding regions of the respective paralogues (Additional file 1).

We first decided to check for nearest relatives/neighbors in databases using phylogenetic analyses of the genes for the putative chitinolytic enzymes (based on predicted protein sequences).

To overcome this difficulty, we incorporated tryptic peptides preceding the cleavage sites based on predicted signal sequences from SMART [ 27], SignalP4.0 [ 28], and cleavage sites annotated in HPRD [ 29] into our analysis pipeline.

C. briggsae genes are designated by contig location, first nucleotide of predicted coding sequence, and strand, based on predicted C. elegans sequence.

Orthologous genes were grouped together, and in each group similarity to the gene in Xoc (if present) was calculated based on predicted amino acid sequence using the needle program of the EMBOSS package [108].

Three distinct groups of EST's were found based on predicted amino acid sequence similarity.