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Based on pre-computed sequence similarity information, a Markov cluster (MCL) algorithm was used to assign proteins into families [ 32].

This method relies on the Markov cluster (MCL) algorithm for the assignment of proteins to families based on pre-computed sequence similarity information.

We suggest that the method based on pre-computed multigenes, as it is done by CAMBer, gives a more reliable estimate of the core genome.

UniRef50 clusters are based on pre-computed sequence clusters of the UniProt database that have at least 50percentt similarity and 80percentt coverage [ 26].

As a result the proportion of the size of core genome with respect to the pangenome substantially depends on the chosen method, yielding higher score for the method based on pre-computed multigenes.

Genes specific to the porcine phylogenetic lineages IV and V were identified using the Phylogenetic Profiler tool (which is based on pre-computed similarities between genes) implemented in the Integrated Microbial Genomes (IMG/ER) system of the Joint Genome Institute [ 46].

Methods We further validate and extend a Computed Tomography (CT) and attenuation map (μ-map) synthesis method based on pre-acquired MRI-CT image pairs.

But weight gain should be based on pre-pregnancy weight.

The seakeeping module computes wave induced ship motions in real-time according to ship's position, heading and speed, based on pre-calculated response amplitude operators.

The ranges of variables were determined based on pre-tests.

to access network resources based on pre-defined credentials.

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