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At each generation, males used for backcrossing were selected based on polymorphic marker analysis and their round spermatids were injected into B6 strain oocytes.
At each generation, males used for backcrossing were selected based on polymorphic marker analysis: low density screening MASP using 74 176 markers distributed uniformly throughout the genome.
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A statistical model will be developed to characterize these QTL based on polymorphic markers, such as single nucleotide polymorphisms (SNPs), genotyped from the avian genome.
New genetic maps, to be devloped based on polymorphic markers identified in this study, will facilitate trait mapping and marker assisted selection.
A total of 453 rats (F2) were selected based on polymorphic markers at Ofil1 (D4Wox22, 37.38 cM and D4Mgh6, 58.99 cM) and Ofil2 (D7Rat35, 6.83 cM and D7Mgh11, 2.30 cM).
Based on polymorphic markers developed using the SLAFseq approach, a super-BSA was conducted to identify candidate genes associated with PMR.
The map was constructed using an F2 population and was based on polymorphic markers developed using the SLAF-seq approach, which allowed the efficient development of a large number of markers in a short time.
Also few isolates were found to be isolated from major groups for instance isolates from BR (Foc_011), HP (Foc_079), GJ (Foc_015, 293, 294) and MP (Foc_263).The PCoA based on polymorphic DArT marker data derived from 110 isolates, confirmed the four different groups found by UPGMA dendrogram.
A set of 799 additional SNP markers was developed based on polymorphic sites identified in contigs and was used to anchor and orient metacontigs to LGs.
The case groups are stratified based on polymorphic status, in most common allele or any polymorphic allele.
Currently, there is one molecular study based on low polymorphic markers (allozymes) that suggests no genetic differentiation within the range of P. pringlei (Fleming et al., 1998).
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