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Additionally, we constructed phylogenetic networks based on pol using SplitsTree 4.10 [ 21].
For evaluating the whole system, Additional file 1 shows the inferred phylogeny of the 268 LTR retroelements investigated based on pol using the NJ method of phylogenetic reconstruction.
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Of the 178 HIV-1 positive infants as determined by DNA PCR on FP-DBS using all the 3 methods (Roche Amplicor® and 'in-house' gag and pol assay), 110 infants had HIV-1 subtype data available based on pol sequence.
This study provides detailed design guidelines for small RNA expression cassettes based on Pol III.
Therefore, glycyrrhetinic acid could be an anti-inflammatory agent based on pol λ inhibition.
In details, 15 and 20 recombinants were detected based on pol and vif-env regions, respectively.
Four phylogenies of 14 extant FVs were constructed based on Pol protein (left column ) and pol nucleotide (right column ) alignments under the Bayesian (top row ) and maximum-likelihood (bottom row ) framework (See Methods for details).
As this is based on using data from 4 HIV-1 pol PCR tests to detection infection, we also examined the sensitivity if we considered the results from just the first (single test), the first two (duplicate) or the first 3 (triplicate) HIV-1 pol PCR tests.
These vectors differ based on the Pol III promoters used to drive the gRNA: AtU6-26p in pBUN401-ZT1, OsU3p in pBUN411-ZT1 and TaU3p in pBUN421-ZT1.
Studies using phylogenetics based on the pol gene of HIV were performed throughout the world to map local HIV epidemics in correlation with transmission pathway, drug resistance, risk behaviour and cluster size.
The first strategy is based on modified Pol II or Pol III promoters, controlled by drug-responsive repressors or transactivators such as Tet-dependent repressors or transactivators [ 3- 17], ecdyson-dependent transactivators [ 18] or the HIV-1 Tat protein [ 19].
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