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CNAT includes a built-in probe-level quantile normalization of signal intensity, which is based on perfect match (PM) probes across the CEL files [26].
Sequenced libraries were computationally assigned to sub-libraries based on perfect match to barcode for EXPRSS data and up to 1 mismatch allowed for NlaIII-DGE data.
This filtering approach did not improve results in terms of known imprinted genes identified in comparison to filtering solely based on perfect match intensity (data not shown).
We obviate the need to consider the mismatch probes when filtering by estimating differential expression using an approach based on perfect match intensities only and using this approach we obtain satisfactory and comparable results to a PM-MM filtering scheme which we implemented as described in Ji et al. [ 4].
An approach developed for bacterial CGH, implemented in a program called TSTEP, identifies deletions using microarray data by scoring probes as absent or present based on perfect match and mismatched probe intensities as well as consideration of neighboring probe data using a sliding window [ 20].
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After sequencing, the sequence reads were separated on sample origin based on perfect matches of the 6 nt multiplex tags.
In Table S1, the reads number for each miRNA is based on perfect matches to known chicken miRNAs in miRBase.
Using the identified seed sequences, we sought targets for the tRFs in the D. melanogaster genome based on perfect matches to 3'UTRs.
We observed that probe level filtering based only on perfect match probe intensities performs comparably to the perfect match and mismatch based approach of Ji et al. One possible reason that these two filtering procedures perform similarly despite fairly different implementations is because the Ji et al. procedure uses the Affymetrix MAS5 algorithm to obtain summary expression intensities.
In general, this algorithm performs target predictions based on perfect and conserved matches between the genes untranslated region (UTR) and the first six nucleotides of the seed sequence.
These filters included: 1) Phred quality (Q) mean scores higher than 30, 2) reads between 15 40 nt in length, 3) adapter detection based on perfect-10 nt match, and 4) removal of reads without detected adapter.
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based on perfect crystal
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based on significant match
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based on perfect link
based on perfect composite
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