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Due to the fact that, in general, mass spectrometric measurement of low abundance proteins is extremely sparse, we developed a metaprotein analytic approach for the analysis of pathway specific phosphorylation changes, based on pathways from MSigDB.
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The software tool CARMEN supports the in silico reconstruction and visualization of automatically derived metabolic networks based on pathway information from the KEGG database and user-defined SBML templates.
Their similarity score is derived from pathway similarity based on pathway components (see reference for details).
Construction of gene networks based on known cellular pathways and expression data from our analysis demonstrated that the network associated with cell death, cell cycle regulation and DNA replication and repair was most heavily affected in this model of disc degeneration.
Interestingly, most of the models are based on the pathway data collated from either the signaling pathway databases or literature mining.
Finally, functional pathways were annotated based on KEGG pathway analyses.
Based on the pathway analysis findings from the microarray study, we hypothesized that PT would suppress recruitment of immune cells to the airways by reducing the expression of proinflammatory cytokines and chemokines.
The sub-network after filtering is shown in Figure 2, which has 95 nodes with 186 interactions derived from six pathways and is organized to show the underlying relevant pathways based on data from MSigDB.
5, 10 The interpretation of the bioinformatics analysis was based on results from KEGG pathways focusing on those most impacted.
To identify important hormone-signaling proteins in sweet orange, we constructed PPI sub-networks related to hormone-signaling pathways, based on information from the Arabidopsis Hormone Database (AHD2.0) [ 41].
We also retrieved KO data from KEGG and then classified enzymes from non-enzymes based on our pathway mapping results.
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