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For example, 36%and47%7% of the metabolic pathways in yeast are confined within only one or two compartments, respectively (based on pathway annotation data in the model of (Duarte et al., 2004).
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This latter option allows for filtering genes based on pathway involvement, chromosomal location, or other annotation information.
To evaluate our annotation process, the annotated unigenes were classified based on pathway analysis which can further help our understanding of the biological functions and interactions of genes.
Compared with the approach of Ramos et al. (2008) which creates an in silico annotation network based on pathways and protein interaction data and maps the gene list of interest afterwards, ClueGO generates a dynamical network structure by already initially considering the gene lists of interest.
First is to process data for ocsESTdb: logical relationships among sequence data, annotation information, pathways and networks are constructed based on unigenes assembled with clean raw ESTs.
SNP pairs were generated based on gene annotation, pathway, and network knowledge, respectively.
Finally, we created gene sets based on metabolic pathways annotated in the KEGG database and Gene Ontology terms associated with gene annotations available at http://www.toxodb.org.
The initial process of metabolic construction for R. centenum was automatic and involved building each pathway based on genome annotation results and the presence of each pathway in the MetaCyc database [ 17].
Based on Ingenuity Pathway Analysis annotation, most of these membrane proteins identified enriched in the conditioned media are related to the processes of cell adhesion and cell movement (Table S4).
We organized functional gene subsets based on gene annotation and pathway information.
In addition, we created a metabolic pathway database based on the annotation information of the assembled cauliflower unigenes using the Pathway Tools [ 40].
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