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In each assay, controls for specificity of cytokine stainings based on parallel staining studies omitting the primary antibody or using primary isotype-matched immunoglobulin (Ig) of irrelevant antigen specificity at the same concentration as the cytokine-detecting antibodies were included.
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Most computing processes are optimized based on parallel programming.
ALDHhigh positivity was selected based on staining parallel cells with DEAB inhibitor of ALDH activity (Additional file 2: Figure S2).
The interpretation of cytoplasmic PAK1 expression was based on staining intensity and staining extent.
EpCAM staining was evaluated using Allred scoring based on staining intensity and number of cells staining positively.
Annexin A7 protein expression was quantified based on staining intensity and uniformity of nuclear/cytoplasmic staining.
Although VIP interneurons were most active based on FOS staining, they were strikingly non-responsive transcriptionally.
Morphometric analysis (B,C,D) performed based on CD31 staining images (A).
In vitro mineralization was evaluated based on histochemical staining and quantification of the hydroxyapatite content of the extracellular bone matrix.
The study is based on appropriate staining methods and protocols developed in our laboratory.
Micronuclei were scored based on DAPI staining.
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