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We used an estimator based on pairwise sequence distances similar to one defined previously, that is relatively fast to compute and has almost the same statistical power as the widely used maximum likelihood estimator [ 66].
The secretory isoforms of human, bovine and mouse PAP are ∼80% identical to one another at the amino acid level (based on pairwise sequence comparisons), suggesting they might have similar antinociceptive effects in vivo.
Clustering all our P450s genes was also performed based on pairwise sequence percentage identity using the complete linkage algorithm [ 12].
First, based on pairwise sequence comparisons and protein threading results, the sequences from Oculina and Tachypleus are highly similar.
However, rather than constructing synteny blocks by considering all input sequences, RACA constructs synteny blocks based on pairwise sequence alignment against only the reference genome.
Histograms based on pairwise sequence comparison showed that NP disjointed most between human and avian influenza viruses, followed by PA and PB2.
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a Minimum proportion of sequence similarity based on BLAST's pairwise comparisons.
Bioinformatics methods for ortholog identification are commonly based on pairwise protein sequence comparisons between whole genomes.
Phylogenetic analyses were performed using a distance method, based on pairwise nucleotide sequence alignments for the core genome among all isolates.
Neighbor-joining trees based on pairwise nucleotide sequence distances were inferred by using PHYLIP (33 ) and plotted by using the APE package of the R statistical package (34 ).
For example, a five-member gene family could have been generated through four gene duplication events, although BLASTP would yield ten gene duplicates pairs based on pairwise comparisons of sequence similarity.
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