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To support these data, Bestkeeper [ 19], another Excel-based tool based on pairwise correlation, was performed and showed similar results concerning the best reference genes for Brachiaria (data not shown).
The average expression correlation (Co-expression as calculated by Pearson correlation coefficients, hereafter termed PC values) of miRNAs was examined based on pairwise correlation calculations of miRNA expression profiles obtained for 26 different organ systems and cell types [ 31].
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Changes in metabolism were analyzed further, using a metabolic network approach based on pairwise correlations between analyte levels in all genotypes and treatments combined (see methods).
An unsupervised correlation similarity matrix was built based on pairwise correlations between genes.
Correlation networks were constructed based on pairwise correlations between all genes of a given dataset.
We incorporated SNPs up to a distance of 10 and 20 Mb to measure haplotype blocks based on pairwise correlations.
Moreover, even though there are a few methods available for network construction with microbiome data [ 28– 32], most methods for network analysis are based on pairwise correlations (or distance) and ignore high-order correlations.
For methylation tree construction, we first calculated the absolute methylation level in sliding windows of 50 kb with steps of 25 kb across the whole genome for the four samples, and then clustered the samples based on pairwise Spearman correlation coefficients estimated from the above whole genome sliding methylation level matrix.
Correlation heatmap of the genes was generated based on pairwise Spearman correlations of the expression profiles of the cells in the 64-cell stage.
Lineage-specific log2 normalized expression profiles were clustered based on pairwise Pearson correlations.
Secondly, we tested the impact of pairwise river distance, barrier characteristics, log-transformed average watershed position, and log-transformed average habitat width on genetic differentiation based on pairwise matrix correlations.
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