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The number of genes undergoing AS were mainly estimated based on overlapping introns, which does not take intron retention and splice junctions into account [ 65].
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Remaining region centers were classified based on overlapping RefGene introns, exonic coding regions, 5'UTRs (>200 bp from TSS), and 3'UTRs.
The relationship is based on overlapping interests, especially oil, not on shared values.
To avoid counts based on overlapping binding motifs, overlapping pairs were not considered.
In this study, a novel method was developed to construct intron-containing hairpin RNA (ihpRNA) rapidly and efficiently based on Overlap Extension PCR (OE-PCR).
In the second step, overlapping groups are merged based on overlap scores.
Cis-NAT pairs can be classified into three groups based on their overlapping patterns, including 3' end overlap, 5' end overlap and one entirely contained within the other [ 49].
First, CDSs were separated into different classes - based on how many introns they contained - and mean intron length was calculated at each intron position within each class (with a requirement of at least 100 introns in each position).
The intron information (for example, intron number, intron length) was extracted from the gene structure predicted for each BAC sequence, and intron density was calculated based on number of introns per gene.
Because only a few species were found to possess an intron 714 or an intron 870, our co-speciation analyses were only based on introns 723.
It has been reported that orthology is based on evolution of introns in plants [ 57, 58].
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