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The DENV 3′UTR (and correspondingly its sfRNA) is comprised of individual structural elements that, based on our probing data, do not appear to interact with each other to form a higher-order structure.
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In this paper, we report a spectrometry to quantify FRET efficiency based on our home-designed spectral probe system and spectral data-processing procedure.
In order to further demonstrate that the behaviour of the membrane targeting probe 6 relies on it being localised on the extracellular face of the plasma membrane, and also that probes based on our new general sensor scaffold are capable of targeting intracellular organelles, we also examined probes 9b and 10b which were designed to target specific organelles in cellulo.
Based on our analysis, total of 4,015 probes were identified with differential expression between BT×623 and Keller.
Based on our measurement of the F1 probe hybridization rate (6 × 10 M−1s−1, Figure 1C), T hybridization = ∼0.33 s (at the 500 nM probe concentration used in the experiment).
With Nimblegen microarrays constructed with the newly designed probes based on our fuzzy gene selection as described in Material and methods, we examined the expression profiles on ICRs cohorts for consistency with predicted histologic grade.
Based on our in-situ stressing AFM, Kelvin probe force microscopy potential imaging revealed the evolution of electrical potential across the junction along with the PID stressing time, which provides vital information to further study the PID mechanism.
The reason that we chose these two dual labeled, self quenched probes is based on our understanding on the nature of FMCA.
Based on our previous study [ 11], the probes were selected from the 46k-microarray (University of Arizona).
Based on our IRAK-M-DD model we probed potential interactive residues via structurally conservative mutagenesis of full-length human IRAK-M (Additional file 2: Table S1).
Based on our quality criteria, 48%% of all probe sets in the murine genome were found to have a "high" quality annotation on the microarrays used in this study.
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