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Animals receiving the Ad5 vaccine were immunized three times, whereas the DNA-vaccinated animals were immunized up to four times based on optimized protocols.
The procedures of whole blood culture are based on optimized protocols developed during pilot studies.
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The best cut-off value was selected based on optimized Youden index.
A newer approach is to institute goal-directed therapy based on optimized flow parameters.
This analysis was developed by measuring similarities among our three clusters based on optimized F ST -based measures.
The primers sets used in this study were based on optimized and validated primers from PrimerBank© (Table 1).
In all experiments reagent amounts were scaled according to surface area of the specific culture dishes used, based on the optimized protocol listed for 24-well plates.
To assess the frequency at which RNA-RNA interactions recovered by CLASH were formed in vitro following cell lysis, lysate obtained from the crosslinked HEK cells expressing PTH-AGO1 was mixed with an equal quantity (measured by RNA content) of yeast cell lysate, prior to CLASH analysis based on the optimized protocol (E4).
HPLC condition was optimized based on the optimized extraction method.
A simplified approach based on an optimized single column protocol is used to design the open-loop SMB.
Our SYBR Green RT-qPCR assay is based on an optimized and validated protocol and is an easily reproducible and reliable tool specifically designed to investigate immune response in ruminants.
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