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Cells were segmented based on nucleus size (set at 5 μm) and fluorescence intensity.
Nuclear staining enables cell coordinates to be determined from fluorescent images based on nucleus size and brightness.
PZ1444 expresses nuclear LacZ in ISCs and cap cells, which can be distinguished based on nucleus size and location (Xie and Spradling, 2000).
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Cells were classified as CTCs based on size, nucleus to cytoplasm area ratio, and nuclear irregularities.
Hoescht staining was used to identify neurons (based on location, size of the nucleus and the presence of several nucleoli) prior to bright-field counting of the proportion of neurons expressing LacZ.
We describe (Results - Cell Counting Algorithm section) that our algorithm uses the Matlab® command round by which we account for discrepancies in nucleus sizes based on ratios (discrete nucleus surface area/representative nucleus surface area).
This suggests that the budding yeast nucleus comfortably accommodates the yeast genome based on the size of our simulated chromosome structures.
*Apparent size based on LIZ GeneScan™500 size standard.
Size your tank based on the ADULT size of the fish, not their CURRENT size.
Rates vary based on yard size.
The packet size varies based on cwnd size.
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