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We selected fast evolving genes in the syntenic segments based on nucleotide substitution rates.
Various phylogenetic studies of flaviviruses (6– 8) have discussed the time period when flaviviruses have emerged or diverged, with estimates based on nucleotide substitution rates.
This result is consistent with a previous rough estimate (~50 Ma) based on nucleotide substitution rates in the small ribosomal subunit RNA [ 45].
Corrected pairwise genetic distance between Helicoverpa species and Heliothis virescens based on nucleotide substitution model (HKY+G) selected by MODELTEST 3.7.
The ages of NIRVs have turned out to be much older than those age estimates from molecular clocks based on nucleotide substitution rates.
Methods to do such removals include ORF detection [ 1, 9, 16, 25], BLAST to identify homologs of protein coding genes [ 25], domain based searches such as Pfam [ 9, 25], and predictions of coding potential based on nucleotide substitution frequencies given sequences from multiple species.
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For each island, we calculated the number of haplotypes based on nucleotide substitutions (h), number of private haplotypes (ph) and haplotypic diversity (H) [41].
Genealogical relationships among haplotypes based on nucleotide substitutions were inferred using the statistical parsimony algorithm [35], as implemented in TCS 1.21 [36].
For the three cases of incongruence, TCS prediction was considered the most reliable because it is based on nucleotide substitutions.
Furthermore, the RIBOSUM 4 × 4 matrix is based on nucleotide substitutions in single-stranded regions whereas we used it as a general substitution matrix.
Polymorphisms based on nucleotide substitutions were detected by the Cleaved Amplified Polymorphic Sequences (CAPS) approach [ 45] or derived CAPS (dCAPS) [ 46].
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