Sentence examples for based on nucleotide sequencing from inspiring English sources

Based on nucleotide sequencing of the expression vectors produced, one clone (E. coli BL21 DnaK) was selected for expression of the recombinant protein.

Further genotypic classification is based on nucleotide sequencing of the ompA gene, which encodes the major outer membrane protein and is the target of serotype classification.

Several techniques, based on nucleotide sequencing targeting different genes, have been employed in attempts to separate isolates of F. psychrophilum, and with highly variable results [ 28– 30].

Current genetic typing of ASFV isolates is based on nucleotide sequencing of the p72 capsid protein gene (B646L) and/or amplification of full-length polymorphisms of various genomic regions (12, 13 ).

Based on nucleotide sequencing of the C/prM and E genes, four genotypes of JEV have been identified; the complete genomes of representatives of each genotype have been sequenced.

Genetic typing was based on nucleotide sequence differences in cDNA obtained by direct one step RT-PCR amplification of the nucleocapsid (N) gene fragment from the samples.

These primers were designed based on nucleotide sequences and intron positions obtained from the DDBJ (http://www.ddbj.nig.ac.jp/).

Primers specific for PMeV were designed based on nucleotide sequences of the viral dsRNA obtained using a RT-RAPD approach.

Sequence was aligned using combination of NCBI GenBank and ribosomal database project and a distance Matrix based on Nucleotide Sequence Homology (Using Kimura-2 Parameter) was constructed.

Ongoing genomic diversification of these begomoviral-satellite complexes was evident based on nucleotide sequence alignments, and analysis of single nucleotide polymorphisms, both factors that created new challenges for primer design.

A pair of primers was designed in an attempt to amplify outer membrane protein (OMP) gene of Fusobacterium necrophorum based on nucleotide sequence of the OMP of Fusobacterium nucleatum.