Sentence examples for based on nucleotide sequence from inspiring English sources
Genetic typing was based on nucleotide sequence differences in cDNA obtained by direct one step RT-PCR amplification of the nucleocapsid (N) gene fragment from the samples.
A pair of primers was designed in an attempt to amplify outer membrane protein (OMP) gene of Fusobacterium necrophorum based on nucleotide sequence of the OMP of Fusobacterium nucleatum.
Ongoing genomic diversification of these begomoviral-satellite complexes was evident based on nucleotide sequence alignments, and analysis of single nucleotide polymorphisms, both factors that created new challenges for primer design.
In this assay, the primers were designed and custom-synthesized based on nucleotide sequence of VP2 gene of CPV 2. A standard curve was plotted using 10-fold serial dilution of standard plasmid DNA and Ct value.
Sequence was aligned using combination of NCBI GenBank and ribosomal database project and a distance Matrix based on Nucleotide Sequence Homology (Using Kimura-2 Parameter) was constructed.
Expression microarrays use probes targeting specific genes based on nucleotide sequence complementarity to quantitatively measure mRNA levels for tens of thousands of genes.
However, based on nucleotide sequence analysis of the rpoB gene isolated from RifR mutants, this behavior of DinB does not appear to contribute significantly to H2O2-induced mutations in P. aeruginosa (Table 2 & Fig. 1).
Shown in Figure 5 is an unrooted phylogeny based on nucleotide sequence alignments (see Supplemental File S4 for the entire alignment and the model and parameters used for phylogenetic reconstruction).
Based on nucleotide sequencing of the expression vectors produced, one clone (E. coli BL21 DnaK) was selected for expression of the recombinant protein.
Figure 1: Bayesian phylogenetic analyses based on nucleotide sequences of the complete GP, NP, L and Z segments of LASVs showing the placement of the virus strains isolated in this study in comparison to other sequences representing the members of LASV lineages I V.
