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Phylogenetic trees were also constructed based on nucleotide polymorphisms within the aligned sequences using the CLC Free workbench (http://www.clcbio.com/) using the unweighted pair-group with arithmetic mean method with 1,000 replicates.
Therefore we disregarded indels, and redistributed the reads to other clusters based on nucleotide polymorphisms only.
This is further corroborated by a recent study based on nucleotide polymorphisms that showed that genes with high expression level and breadth were more conserved than tissue-specific genes in P. glauca (Pavy et al. 2013).
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In the present study, we aimed to reconstruct the demographic histories of two peat moss species based on nucleotide polymorphism data.
In this method, N e s is compound parameter, but following Petrov et al. [ 18] we assume values for N e based on nucleotide polymorphism data.
The number in italics below the value for each test is the upper limit of the 95% confidence interval, calculated by coalescence simulation based on θπ and free-recombination, n = number of sequences compared, S = number of segregating sites, π = nucleotide polymorphism and θπ = theta (an estimator of genetic variation) based on nucleotide polymorphism.
Polymorphisms based on nucleotide substitutions were detected by the Cleaved Amplified Polymorphic Sequences (CAPS) approach [ 45] or derived CAPS (dCAPS) [ 46].
Type M was further divided into 33 subtypes based on single nucleotide polymorphisms, single nucleotide deletion (V12), double nucleotide deletion (V19) and single nucleotide insertion (V20) (Tables 3, 4).
We have used association studies based on single nucleotide polymorphisms, first with candidate genes and then in a genome-wide scan of 266,000 single nucleotide polymorphisms, to search for the putative predisposing genes.
Identification of allele-specifically expressed genes has relied on RNA-based technology to infer differentially expressed alleles based on single nucleotide polymorphisms (SNPs).
Therefore genotyping based on single nucleotide polymorphisms (SNPs) can indicate their origins, spread and phylogeny.
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