Sentence examples for based on nucleotide differences from inspiring English sources

Extracted DNA was amplified by a nested pan-alphaherpesvirus PCR as described previously [ 17, 21], which has been widely used for identification of different ruminant alphaherpesviruses based on nucleotide differences in a highly conserved gene coding for glycoprotein B. CvHV2 (Finnish isolate, Salla82) was used as a positive control and sterile water as a negative control.

Secondly, the MOTU-specific PCR primers (Cm simile-specific ITS and Cm verum-specific ITS, Table 1) were used to differentiate the simile and verum MOTUs based on nucleotide differences in the ITS gene region identified in DNA sequence alignments (Fig. 1, Additional file 1: Table S1).

Mammalian HEV falls into four major genetically distinct genotypes based on nucleotide differences [ 4- 6].

To estimate genetic differentiation based on nucleotide differences we used MEGA 4.0 [ 72] to calculate uncorrected p-distances and TN distances [ 73].

The insertion time of CR elements was calculated based on nucleotide difference between the 5′- and 3′-LTRs of LTR retrotransposons, an approach pioneered by SanMiguel et al. (1998).

In order to confirm the phylogenetic position of CC75 relative to typical S. aureus and S. epidermidis, we constructed a tree based on nucleotide divergence of the core genes and a second tree based on differences in genome content.

Indeed, in sample CAR1 the number of OTUs drops from 5,600 to ca. 2,600 when phylotypes are clustered based on one nucleotide difference (accounting for ca. 0.8% sequence similarity).

Genetic typing was based on nucleotide sequence differences in cDNA obtained by direct one step RT-PCR amplification of the nucleocapsid (N) gene fragment from the samples.

Levels of genetic differentiation were measured by the fixation index FST, based on nucleotide frequency differences [64].

Genetic typing was based on nucleotide sequence differences in complementary DNA (cDNA) for the nucleoprotein gene as aligned with sequence for the Pasteur vaccine strain of rabies virus (7 ).

The collapse of detected OTUs when comparing unique tags with OTUs based on two nucleotide differences (roughly 1.5% difference in primary structure), is remarkable: in the same sample (FV1) up to 6.3 times more unique OTUs were detected compared to OTUs2 nt.