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In this manuscript, we devised more stringent criteria based on nucleotide conservation to extract highly conserved miRNA families.
Assuming that regulation of miRNA expression is conserved among mammals, we used the PhastCons evolutionary conservation track that is based on nucleotide conservation among placental mammals (http://hgdownload.cse.ucsc.edu/goldenPath/hg18/phastCons44way/) to quantify evolutionary conservation of the predicted TFBSs.
Similar(58)
The majority of the introns identified in the T. vaginalis genome were found using a sequence-pattern search strategy based primarily on nucleotide conservation observed between the intron boundary sequences of the first identified T. vaginalis intron and the G. lamblia ferredoxin intron.
Chen, W., Yang, H., Feng, P., Ding, H. & Lin, H. iDNA4mC: identifying DNA N4-methylcytosine sites based on nucleotide chemical properties.
This reaction is based on nucleotide complementarity [19].
Bisulfite-sequencing was based on nucleotide sequences in electropherograms.
These analyses were based on nucleotide sequences.
15, 16 Methods based on nucleotide statistics were also introduced.
Other methods based on nucleotide statistics are sensitive to nucleotide periodicity in CDS.
The analysis was based on nucleotides 10 2241.
(3) Based on sequence conservation: If nucleotides at the bind site are conserved or nearly conserved across virus strands in different years, additional reward score is given.
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