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For laboratory tests, toxicity grading was assigned using normal reference ranges based on a similar local pediatric population with the exception of absolute lymphocyte counts, which were based on normal values in Ugandan children [28].
Based on normal values of the laboratory (mean [95% CI]: 92.7 [83.4–101.9] ng/ml) [17], [18] we calculated that we could detect at least a 26% change in patients (n = 85) as compared to controls (n = 29) with 81% power and p-alpha (2-tailed) = 0.05, by the T test.
The 95th percentiles for the YSR and CBCL were based on normal values obtained from a Chinese reference population in Hong Kong [ 20], while the 95th percentile for the MHI were based on the current sample of Shanghai students.
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A group of 25 healthy volunteers was selected as control, based on normal hematological and biochemical values, and no history of kidney or inflammatory diseases, in order to better define the changes occurring in HD patients.
Protein abundance data were calculated based on normal spectral abundance factor (NSAF) values as described previously [ 7].
An MS method similar to ours had been used in a study of patello-femoral OA [ 27] but without the use of threshold values based on normal bone marrow.
Normal biological parameters values were based on the normal values for our laboratory: fasting glucose: 3.3 to 6.1 mmol/L; HbA1c: 4 to 6%; total cholesterol: 3 to 5.2 mmol/L; triglycerides: 0.3 to 1.7 mmol/L; NT-pro BNP < 300 pg/mL; CRP < 10 mg/L.
For this analysis, FVC was converted to a percentile value for each participant based on normal reference equations.
*Missing/unknown values set as failures; a95% CI based on normal approximation to the binomial.
Laboratory values were presented as medians with interquartile ranges and were dichotomized into normal or abnormal based on normal ranges for children and adults (Supplementary Table 1).
The general idea is to forward simulate the network, based on the "normal" values for each variable (the value with high probability given its parents).
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