Limits for the quadrant markers were set based on negative populations and controls isotype (data not shown).
Locus counts were assessed for differential expression by applying an exact test based on negative binomial distributions of count data as implemented in the R package edgeR [ 19].
CpG sites with ≥0.05 "Detection p value" (computed from the background based on negative controls) were eliminated from the data for further analysis, leaving 24,949 valid for use with the 16 samples tested.
Generalized linear models based on negative binomial distributions were fit to the data using edgeR, and the model includes treatment and replicate effects.
Based on the negative data from the KyberSept trial [ 165], a Cochrane analysis [ 4], and subgroup analyses of several trials [ 72, 87, 167], we do not recommend the routine use of antithrombin as treatment of neutropenic sepsis in the absence of disseminated intravascular coagulation (DIC; DI).
The experimental data here are based on negative-stain EM images on monolayers of egg PC:cholesterol (45:55%), incubated at 37°C.
(See: "Lower Start On Negative Data").
Based on the above negative data, we decided to expand our studies to stress responses and apoptosis.
