Sentence examples for based on negative controls from inspiring English sources
The error rate (3%) was calculated based on negative controls and repeated samples [47].
The calculated error rate was approximately 3% based on negative controls and replicated samples.
CpG sites with ≥0.05 "Detection p value" (computed from the background based on negative controls) were eliminated from the data for further analysis, leaving 24,949 valid for use with the 16 samples tested.
The comparison of the gene expression between the two strains was performed as follows: Microarray data were imported into the GeneSpring GX version 7.3.1 software (Agilent Technologies, CA, USA) and initial background subtractions were performed based on negative controls and intensity-dependent (Lowess) normalization using the replicate cross-gene error model implemented in the software.
The positive threshold was determined based on negative controls as described in the manufacturer's protocol.
However, as the cutoff value of 200 was determined based on negative controls on the chip to match some optimum ratio of FN to FP, and to our knowledge, almost all published works utilizing this microarray dataset used the cutoff 200, a liberty given to the lower cutoff value for reducing FN should not be encouraged.
Based on negative control measurements, unspecific background intensity was defined.
The data was processed with Beadstudio software (version 3.1.3, gene expression module 3.3.8) including the calculation of detection p values based on negative control bead signals.
