Sentence examples for based on negative control from inspiring English sources

Gating was implemented based on negative control staining profiles.

Based on negative control measurements, unspecific background intensity was defined.

Quadrants were determined based on negative control staining with a corresponding isotype antibody.

Gates were based on negative control signals, and plots generated using FlowJo 8.8.2 (Tree Star, Inc., San Carlos, CA, USA).

The data was processed with Beadstudio software (version 3.1.3, gene expression module 3.3.8) including the calculation of detection p values based on negative control bead signals.

Raw data were background-subtracted and normalized using the "rank invariant" algorithm and then filtered for significant expression based on negative control beads.

The error rate (3%) was calculated based on negative controls and repeated samples [47].

The calculated error rate was approximately 3% based on negative controls and replicated samples.

CpG sites with ≥0.05 "Detection p value" (computed from the background based on negative controls) were eliminated from the data for further analysis, leaving 24,949 valid for use with the 16 samples tested.

The comparison of the gene expression between the two strains was performed as follows: Microarray data were imported into the GeneSpring GX version 7.3.1 software (Agilent Technologies, CA, USA) and initial background subtractions were performed based on negative controls and intensity-dependent (Lowess) normalization using the replicate cross-gene error model implemented in the software.

The positive threshold was determined based on negative controls as described in the manufacturer's protocol.