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Based on mutant phenotype, TKL1 is presumed to contribute to the supply of AAAs; the function of the ScTKL2 isogene presumed complementary of TKL1 remains unclear.
RNA was extracted from approximately 30 pooled esco2+/+ (AB), esco2 sibling (sib; containing WT and heterozygous embryos) or esco2 m/m (based on mutant phenotype at 24 hpf) embryos using Trizol reagent (Life Technologies), according to the manufacturer's suggested protocol.
In such cases, we would want to annotate all genes reported to be involved in the process, based on mutant phenotype evidence, with both GO and the corresponding phenotype terms.
In our screen of cell surface and secreted proteins, we identified 9 additional effectors, based on mutant phenotype, as well as highlighted 14 other genes that may also play a role in supporting mesoderm migration.
Transposable elements (see Glossary), first described by Barbara McClintock (McClintock, 1950), continue to be an important tool for cloning genes based on mutant phenotype, and their use has provided novel insights into epigenetics (Lisch, 2012), such as the relationship between transposon activity and DNA methylation (Chandler and Walbot, 1986).
Patterns involving sequences of arbitrary length can be easily modeled in OWL using nested class expressions for example, an evidence chain involving sequence similarity based on mutant phenotype can be described with an expression 'based on' some ('sequence similarity' and 'based on' some 'mutant phenotype').
Similar(53)
Based on mutant phenotypes, magnetosome alignment is thought to occur via the interaction of the MamK cytoskeleton with MamJ, a protein located in the magnetosome vesicle.
Based on mutant phenotypes, differential roles in nuclear migration have been ascribed to the motor proteins.
Not all our reporter transgenics, however, were expressed in patterns expected based on mutant phenotypes of the genes examined.
PomBase curators have begun reviewing GO annotations based on mutant phenotypes, to remove those that are known to represent indirect 'downstream' effects.
Many founding members of the SUN and KASH domain protein families have initially been identified in model organisms based on mutant phenotypes affecting nuclear anchorage or migration.
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