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Although such a binding mode with Hfq has not been reported, this sort of behavior was previously predicted based on mutagenesis data [28].
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The intracellular concentration of SAM has been increased by a strain improvement based on mutagenesis.
Finally, basing on the mutagenesis data obtained for toxin γ (Naja nigricollis) [45], [46], it was suggested that the residues of the M site should be involved in the interactions with phospholipids [50].
For HbHNL, this role was unequivocally ascribed to Lys236 based on structural and mutagenesis data.
Based on site-directed mutagenesis data, we established that three sites under positive selection were involved in the functional shift leading to the type-A feruloyl esterase function.
Based on thermodynamic data, site-directed mutagenesis and UV deference spectroscopy, it was concluded that pKa1 corresponds to GSH carboxylates, whereas the pKa2 has a conformational origin of the protein.
Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the generation of small and smart mutant libraries requiring minimal screening.
13Estimates based on fragmentary data.
Every line is based on research data.
"Everything was based on false data".
That report was based on 2006 data.
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