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Dowton et al.26 proposed "next-generation" DNA barcoding based on multilocus data in which they incorporated multispecies coalescent species delimitation.
As there is no proper approach to directly assess the quality of alignments of such large sizes, we compared the resulting phylogenies to previously published phylogenies that were based on multilocus data of genes evolving at slower, more appropriate rates.
Although most species are monophyletic in a tree based on the AFLP data, methods for delimiting genotypic clusters based on multilocus data alone did not recognize all these species.
We checked the morphological species delimitation using a tree and a network based on AFLP markers [ 23] and tested the efficiency of several approaches for delimiting genotypic clusters based on multilocus data without a prior knowledge of the number of species.
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STRUCTURE uses Bayesian clustering method to assign individuals probabilistically to populations based on multilocus genotype data.
To investigate the possibility of a separate migration wave from Africa to Oceania, we can consider three simplified scenarios concerning human dispersal from Africa to Oceania that have the potential to be distinguished based on multilocus population-genetic data (Fig. 6).
As the underlying data are based on multilocus microsatellite genotypes, an individual is characterized by a series of pairs of microsatellite repetitions at k loci with k = 9.
Recently, Jurjevic et al. [ 58] undertook the study of section Versicolores and provided useful phylogenetic data on species of this section based on multilocus DNA phylogeny, but still the inclusion of these species in this section has not been resolved.
We therefore decided to investigate the possibility of constructing a molecular epidemiology tool based on multilocus sequence analysis (MLSA) with PCR amplification of various loci followed by sequencing.
These two pairs of sister-species were used as test cases to evaluate how gene flow and shallow divergence times might affect species discrimination and identification based on multilocus barcoding.
A 1999 report described an EAEC phylogeny based on multilocus enzyme electrophoresis (MLEE) [19].
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