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Where scores were ambiguous, decisions were based on morphology at the light-microscopy level.
Further studies are needed to confirm our data, mainly based on morphology, at immunohistochemical level by the study of apoptotic marker proteins and at molecular level using immunoblotting analyses of tissue mRNA/protein expression and of oxidative stress markers.
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For example, an image acquired at 740 nm can be examined to see that the endothelium is abnormal based on morphology, however, an image at 800 nm would need to be compared to appreciate the overlying collagen structure of this region.
Individual distal tubule segments were identified based on morphology in a dissection microscope at ×25 40 magnification by their appearance and dimensions.
Individual proximal tubule segments were identified based on morphology in a dissection microscope at × 25 40 magnification by their appearance and dimensions.
This demonstrates the difficulties and uncertainties in detecting hybrids solely based on morphology, which could be particularly difficult at low levels of introgression.
Most of our identifications were made, at least preliminarily, based on morphology and anatomy.
Identification at the species level based on morphology alone is difficult.
Retinal ganglion cells (RGCs) are classified into at least 10 15 types based on morphology and/or physiology in mammalian species [1] [3].
In total, 28 ascidians were identified at the species level and another 9 at the genus level based on morphology and genetic barcoding.
They were incubated for 48 hours at 30°C and single colonies were selected based on morphology and re-streaked on fresh agar plates.
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