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Methods for delimiting species based on molecular sequence data can be broadly classified in two categories: tree-based, and non-tree-based [ 1].
Note: Based on molecular sequence data, Athyrium, Cystopteris, Woodsia removed to Woodsiaceae to preserve a monophyletic Dryopteridaceae.
Phylogenetic relationships are based on molecular sequence data from Duda and Kohn (2005).
Biodiversity assessments based on molecular sequence data may be a complement or an efficient alternative to traditional morphology-based approaches.
Another model, based on molecular sequence data, places Ginkgo with the Cycadales [ 10- 12].
Phylogenetic studies based on molecular sequence alignments are expected to become more accurate as the number of sites in the alignments increases.
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Here, we preliminary consider our samples as belonging to Y. cf. dalliana, but this hypothesis should be checked in a future based on molecular sequences of the topotypes from Lashio.
Calibration of demographic histories based on molecular sequences is notoriously difficult, particularly when specific rates of molecular evolution are unavailable [ 57, 58].
However, most of these studies were based on molecular sequences, which are fast evolving and of limited value for deep evolutionary explorations.
We emphasize that Netdis is not proposed as a competitive method for the generation of phylogenetic trees for biological species; existing techniques based on molecular sequences already address this particular problem comprehensively.
Considering the increasing interest in precisely mapping out the evolutionary steps for C4 photosynthesis and the importance of the Flaveria genus in studying C4 evolution, it is pertinent to re-evaluate the phylogeny of Flaveria using the information-rich data based on molecular sequences that has recently become available [ 30].
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