These methods, however, are based on microscopic inspection and cell counting by different researchers and, as a consequence, they are fastidious, time-consuming and error-prone.
Primary assessment of NPC is currently based on microscopic examination of cells and tissues.
Based on microscopic examination, the average cell size of Ao38 cells appeared similar to that of High Five cells although the general morphology shows slight differences in cell shape, with High Five cells exhibiting slightly longer cell extensions.
In addition, in DBA patients, the TUNEL method was performed according to the manufacturer's protocol (kit ApopDETEK, DAKOCytomation, Enzo Diagnostics Inc., Farmingdale, NY, USA) on the cells isolated from erythroid colonies and the percentage of apoptotic cells was determined based on microscopic counting of 200 cells.
Although it has frequently been reported that B deficiency leads to abnormal cell wall structure based on microscopic observation, what exactly occurs in the architecture of cell wall under this condition remains unknown.
Survival was evaluated by comparing oocyte morphology upon freeze-thaw with that of fresh cells (based on microscopic observation).
The resultant fractions were assayed for neurite outgrowth in PC12 cells based on microscopic assessment.
The boundaries of the pallium with neocortex on each tissue section, including scars when present, were marked, based on microscopic observations of lacZ and haematoxylin-stained cells, and then measured bilaterally by planimetry (Teledyne Post planimeter; Figure 5, lines A-A′-B′-B).
Historically, Alexandrium species were described based on microscopic observations of morphological features including plate patterns, cell size and shape, and secondary characteristics such as chain formation.
Our results based on microscopic analyses showed that Ehrlichia were contained in the filopodium of macrophages which were transported to neighboring cells.
