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Based on microsatellite data, only one island (Marchena) showed signatures of a genetic bottleneck, where the harmonic mean of the effective population size (Ne) was estimated to be less than 50 individuals during the period between samplings.
A model-based assignment test was performed based on microsatellite data using STRUCTURE 2.0 [ 31].
Consistent with the mtDNA analysis, all but one pairwise FST comparisons based on microsatellite data showed significant values both at the region (microsatellite dataset I) and drainage (microsatellite dataset II) levels.
The program STRUCTURE v. 2.0 (Pritchard et al., 2000) was used to infer the putative number of populations (K) based on microsatellite data.
In the present study we analyse a dataset that presents evident cases of geographical segregation, such as island effects and we are able to show that the proposed methodology spots these islands, based on microsatellite data only and without any further geographical information or evolutionary assumption.
A Bayesian clustering approach, implemented using STRUCTURE version 2.3 software [30], was also used to estimate the population structure based on microsatellite data.
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These estimates suggest an earlier origin for the hybrid taxa than has been previously suggested based on microsatellite divergence data [ 58- 60], but are largely consistent with more recent revised estimates based on EST sequence divergence data (L. Rieseberg, University of British Columbia, personal communication).
One male with high assignment probability to C. elephantopus based on microsatellite genotypic data also possessed a "Floreana-like" mitochondrial DNA haplotype.
Differentiation statistics based on microsatellite allele data are widely used for studying moderately-structured populations [ 61].
Recently, substantial recombination and genetic exchange among strains from the TcII DTU that coexist in the same geographical area was proposed based on microsatellite genotype data [ 19].
These results demonstrate that assignment tests based on microsatellite DNA data can be powerful tools for distinguishing closely related species and support the validity of P. putjatia.
More suggestions(15)
based on seismic data
based on mitogenomic data
based on fossil data
based on prospective data
based on primary data
based on international data
based on robust data
based on morphological data
based on realistic data
based on official data
based on large data
based on anthropometric data
based on phylogenetic data
based on secondary data
based on environmental data
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