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Many studies have selected candidate miRNAs in EVs based on microarray results and validated them by qRT-PCR.
Networks were merged and genes that were not connected to first interactions with RNAP II, or were not expressed differentially based on microarray results, were deleted.
HOXA4 was the most significantly decreased HOX gene in human AAA tissue based on microarray results.
Gene expression ratios are presented of 18 differentially regulated genes involved in G2 to M phase transition based on microarray results.
Based on microarray results we decided to evaluate the generation of ROS and membrane damage in E. coli cells exposed to biomimetic QDs.
Based on microarray results, expression of two inflammation-related genes (Ifit1 and Iigp1) was measured by qRTPCR in the present study.
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*lower estimates of genomic content based on microarray hybridization results and validation methods.
Clustering of these cells based on microarray expression results showed a high correlation between PG-haESCs and control diploid ESCs, but not MFs.
The 21 plausible B. mori miRNAs are listed in Table 1 of Additional file 1. Northern blotting was done based on microarray assay results to confirm the expression of B. mori miRNAs.
Thirdly, like all in silico modelling based on microarray or RNAseq data, results of special interest from GRN analysis need to be confimred using laboratory experiments as we have done here.
Ten of the 12 upregulated genes (as determined by real time qRT-PCR analysis) were also upregulated in the RNA-seq and microarray platforms―only the IL15 and IRF1 genes were not differentially expressed based on microarray and RNA-seq results, respectively.
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