The next generation of reports describing global gene expression patterns based on microarray assays will probably derive from studies of tissue samples from sites of disease activity.
The gene profiling technology based on microarray assays will hopefully allow for a better selection of candidates for systemic adjuvant treatment and for individualized chemotherapy.
Combining a computational method based on sequence homology searches with experimental identification based on microarray assays and Northern blotting, we identified 46 miRNAs, an additional 21 plausible miRNAs, and a novel small RNA in B. mori.
In present study, we set out to mine E3s from the mouse genome and to characterize their expression pattern, subcellular localization, and enzymatic activities based on microarray data and biochemical assays.
A previous 4C assay, based on microarray measurement of amplified 3C products, suggested that interchromosomal interactions did not contribute significantly to the nuclear microenvironment of the β-globin locus [23].
Based on microarray analysis and Taqman miRNA assays we conclude that members of the miR-146 family, which is highly conserved between fish and human, are induced by bacterial infection in zebrafish in a MyD88 and Traf6 dependent manner.
Precise copy number estimation by TaqMan qPCR assays was observed for four prioritized CNVRs, identifying all CNV carriers predicted based on microarray analysis with no false positives (Supp. Table S4, Supp. Fig. S5; false negative rate across regions 1.4%).
Based on microarray hybridizations, nighteen genes representing different functional categories and a range of gene expression values were selected for qPCR assay (iQ5 Real-Time PCR System, Bio-Rad).
The problem of uncovering transcriptional regulation by transcription factors (TFs) based on microarray data is considered.
Many studies have selected candidate miRNAs in EVs based on microarray results and validated them by qRT-PCR.
