Sentence examples for based on methylation status from inspiring English sources
The second category includes methylation-sensitive restriction endonucleases, which distinguish sequences based on methylation status; furthermore methylcytosine could be identified by immunoprecipitation with antibodies or by affinity purification on methyl-binding protein beads.
We attempted to identify some models for differentiating CRC samples from normal samples based on methylation status.
More recent clinical trials have started adding additional stipulations based on methylation status or blood FMRP expression as a way to identify patients most severely affected or likely to respond the drug treatment, although this method has been largely unsuccessful.
To further explore the association between the sputum and serum samples and the tumour sample, logistic regression was used to obtain odds ratios for tumour methylation based on methylation status of the sputum or serum.
Intuitively, we adapt this approach and replace the species tree with a cell lineage tree and the four-state alphabet of DNA with an alphabet based on methylation status.
Primers directed to a particular sequence are designed to detect either the methylated or unmethylated converted DNA and thus selectively amplify the target sequence based on methylation status as in Miller et al. Bisulfite treated DNA was amplified using QuantiTect SYBR Green PCR Kit (Qiagen) in a Rotorgene RG3000 (Corbett Research).
However, when cases were coded based on methylation levels (using as a threshold the median PMR value), the frequency of IBC samples with a high APC methylation status (74%) was significantly increased when compared to non-IBC (46%) (P=0.048; χ).
Percent methylation was calculated based on the methylation status of each cytosine within the two regions sequenced, using 12 to 24 clones per sample.
Overall methylation profiles were classified as methylated or unmethylated based on the methylation status of the three regions (3R classification method).
Two additional filters are based on methylation rates.
A smoking score based on methylation profile was constructed.
