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Nitric oxide (NO) was assessed using the Griess reagent based on methods previously described (Green et al. 1982).
In all cases, the presence of the trx-1 deletion was demonstrated by performing PCR on single-worm lysates, based on methods previously described ([53]; reviewed in [54]).
Social approach behaviours were tested in a modified three-chambered apparatus based on methods previously described (56).
Therefore, SNPs were detected and scored based on methods previously developed for the polyploid oilseed rape Brassica napus [ 21, 28].
The purification of the lysosomal fractions for CMA assays was performed by a modified protocol based on methods previously described (Kaushik & Cuervo, 2008; Malkus & Ischiropoulos, 2012).
These signals were also used to establish step cycle parameters (such as heel contact and stance to swing transition) based on methods previously described [ 4].
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A confocal fluorescence microscope was used to analyze ovule development in rsw4 plants, based on methods described previously [37].
The in vitro ubiquitination experiments were based on methods described previously [ 20, 21] with minor modifications.
Levels of NHE1 at the cell surface were assessed by a surface protein biotinylation method based on methods described previously [ 17, 26].
The first step, based on methods developed previously by Bielas and colleagues, enriches for deletion-bearing molecules and improves mutant specificity (Bielas & Loeb, 2005; Vermulst et al., 2008a).
TC concentrations were measured enzymatically by means of the VITROS CHOL slide technique using the Triton X-100 surfactant, which is based on methods described previously [ 27].
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