Sentence examples for based on membrane integrity from inspiring English sources

Our results also suggest that post-thaw viability (based on membrane integrity assays) not necessarily reflects the quality of the cryopreservation procedure and proper functionality tests must be carried out in order to optimize both post thaw viability/cell yield and in culture performance.

This stain distinguishes live cells from dead bacteria, based on membrane integrity and two nucleic acid stains.

hMSC viability was assessed using the commercially available Live/Dead staining kit (Invitrogen), which differentiates viable cells from dead cells based on membrane integrity.

To determine cell viability of the biofilm based on membrane integrity, glass flow cells were rinsed with PBS to remove any unattached cells and stained with Live/Dead BacLight (Molecular Probes) for 1 h at room temperature.

Following gel polymerization, the hMSC laden hydrogels were cultured under standard conditions, and cell viability was assessed at 24 and 72 h via Live/Dead staining, which discriminates dead cells from viable cells based on membrane integrity.

Results in model membranes are consistent with flow cytometry experiments in Escherichia coli and Staphylococcus aureus using a membrane potential sensitive dye (bis-oxonol) and a nucleic acid dye (propidium iodide), suggesting that the mechanism of action is based on membrane binding and collapse of membrane integrity by depolarization and permeabilization.

The assay of AO EB staining is based on cytoplasmic membrane integrity.

First, we determined the cytotoxicity of SMYGT in HUVECs based on cellular membrane integrity.

Cell viability based on a membrane integrity assay for chondrocytes cultured in PEG hydrogels containing the native or non-native HA binding peptide for 28 days.

Hence, there are two objectives in this study: first, to confirm whether L. leucocephala leachate is phytotoxic to water hyacinth, based on its effects on membrane integrity and tissue respiration; second, to confirm whether any phytotoxicity detected is mediated by suppression of antioxidative defence.

To determine the cytotoxicity of DETC to V79 cells, cell viability was evaluated based on DETC effect on membrane integrity and on enzymatic activity of mitochondrial dehydrogenases using two tests: trypan blue (TB) exclusion assay and MTT test (Fig.  1).