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Results in model membranes are consistent with flow cytometry experiments in Escherichia coli and Staphylococcus aureus using a membrane potential sensitive dye (bis-oxonol) and a nucleic acid dye (propidium iodide), suggesting that the mechanism of action is based on membrane binding and collapse of membrane integrity by depolarization and permeabilization.
We previously established SH2 profiling, a phosphoproteomic approach based on membrane binding assays that utilizes purified Src Homology 2 (SH2) domains as a molecular tool to profile the global tyrosine phosphorylation state of cells.
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Water purification with two membrane systems, based on membrane coating covalent binding versus adsorption, were analyzed and compared with granulated activated carbon made from coal and wood.
Very recently, a "myristoyl switch" mechanism driven by a combination of electrostatic attraction and membrane curvature sensing was proposed based on comprehensive Nef membrane binding data [42].
These observations suggest a model for cell entry based on direct plasma membrane binding enabled by electrostatic interaction between the virus and the cell membrane.
This is explained by the fact that the flexible mobility of erythrocyte membranes is based on the binding of calcium and magnesium with spectrin (the main protein of the cytoskeleton) [ 20].
This family is further subdivided into two distinct classes, EphA (A1 to A10) and EphB (B1 to B6), based on their binding affinities for two membrane-anchored ligand families with the corresponding names of type A (A1 to A5) and B (B1 to B3) ephrins [19], [20].
This work aimed to assess the usefulness of a panbacterial quantitative real-time PCR reaction to quantitate the total bacterial load in chronic wounds treated with Cutimed™ Sorbact™, a novel therapeutic approach based on hydrophobic binding of bacteria to a membrane.
To further illustrate the difficulty of the current classification task of intrafamily separation, a simple unsupervised classification scheme aimed at predicting the membrane-binding behavior of a domain based on the binding behavior of closely related homologs is fashioned.
Cells membrane chromatography (CMC), a chromatographic biological affinity method that uses specific cell membranes as the stationary phase, has been confirmed as a simple, specific, effective screening method based on the binding principle that the recognition and interaction of drug molecules and targets, receptors, channels on the cell membrane [ 14].
However, previous studies identified the protein Scyl1 binding partner 1 (Scyl1basedbased on its binding to Scyl1 [30].
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