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Previous studies have assigned exposure based on measured nitrate in drinking water instead of estimating daily ingestion.
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We calculated higher cellular Mo levels in N2-fixing cultures (from 0.4 to 1.5 fg Mo per cell) than in cultures grown with nitrate (from 0.1 to 0.6 fg Mo per cell) based on measured Mo concentrations in digested cell pellets and cell counts.
Therefore, we could not determine a flow-weighted measure of distribution water quality for each CWS based on the nitrate level measured in samples from each contributing source.
The activity of nitrate reductase was determined based on measurements of the amount of nitrites produced from nitrates during the enzymatic reaction.
On the other hand, the ratios [Na+]/([Cl−]+[NO3−]) for fine mode aerosol were quite lower than 0.85 indicating the overestimation of nitrate, i.e. the measured nitrate was not totally the nitrate from sea-salt but also from some other sources.
Food and water consumptions were monitored daily, and nitrate intakes were calculated from measured nitrate contents.
Since the major focus of this study is on nitrate contamination, a most commonly used irrigation index, Wilcox diagram (after Wilcox 1955), is plotted based on the measured parameters.
The summary measure based on mean nitrate in all sources within the respective distance was consistently most strongly correlated with homes' tap water nitrate.
NO production was assessed by measuring the nitrate and nitrite concentrations according to the commercial kit from Cayman Chemicals, based on the nitrate conversion to nitrite using nitrate reductase followed by the Griess reaction.
(Both Autoliv and TRW now use a propellant based on guanidine nitrate, a compound that is less sensitive to moisture and temperature swings).
This is based on reducing nitrate to nitrite by a copper-cadmium reductor column.
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