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Phylogenetic trees were reconstructed separately for ITS and trnL-F based on maximum parsimony (MP) and maximum likelihood (ML) criteria implemented in MEGA5.
All three analyses yielded identical tree topologies, which were highly congruent with our previous findings based on Maximum parsimony (Figure 2, Figure 3A, Figure S1).
While many bacterial genomes exhibit high levels of genetic recombination, S. aureus has previously been shown to be highly clonal [9], [15], and therefore, a minimal spanning network based on maximum parsimony analysis was also conducted.
Phylogenetic network of miR-17 family based on maximum parsimony option indicated complex structure with many median vectors (red circles) that were lost ancestral or potential mature miRNA sequences (Figure 1A).
Phylogenetic analyses based on maximum parsimony were performed using PAUP* 4.0b10 [ 51].
The phylogenetic analysis based on Maximum Parsimony (MP) tree was largely consistent with the phylogenetic relationship of the NJ tree.
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Phylogenetic analyses were performed based on maximum-parsimony (MP) and maximum-likelihood (ML) criteria.
Based on maximum-parsimony (MP) bootstrap analysis, most relationships were strongly supported (≥95%).
Phylogenetic tree reconstructions based on maximum-parsimony (MP), neighbor-joining (NJ), maximum-likelihood (ML) and Bayesian algorithms revealed various strongly supported clades, which corresponded to genera, species and subspecies.
Using other alveolates (i.e. ciliates and Apicomplexa) as outgroup species, phylogenetic analyses based on Maximum Likelihood, Maximum Parsimony, and Neighbor-Joining supported Gyrodinium as a separate lineage.
Reconstructed ancestral rhodopsins at each node based on Maximum Likelihood and Maximum Parsimony approaches gave similar results, with most nodes having the 13 key amino acids of the mammalian consensus sequence (see Table S4 and Figure S2, Supplementary Material online).
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