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Genetic mapping offers us a powerful tool to display the chromosomal segments that unevenly transmit to the offspring based on marker segregation distortion [ 9].
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Base on marker segregation distortion and linkage analysis in the progeny of a hybrid, we can track the genomic regions that act as introgression barriers.
We inferred a linkage map based on the marker segregation data from 201 F2 hybrid male embryos after discarding individuals for which more than 20% of the markers failed to amplify.
The single-tree genetic maps are based on segregation of markers in haploid megagametophytes of maternal trees and do not take into account the segregation or recombination of markers in the paternal trees.
We created a molecular linkage map of A. majus based on segregation of markers in the F2 population of two inbred lab strains of A. majus.
A genetic map based on segregation of markers in a population of 572 F2 plants from the cross Ae. tauschii AL8/78 × Ae. tauschii AS75 [ 30] was used as a backbone for the development of diversity maps.
This iterative process was considered complete when no additional markers were removed based on duplicated marker patterns or unusual segregation patterns.
However, the amount of distortion was likely underestimated, because we initially selected loci to map based on their segregation profile and markers not meeting that criterion were not included in building the map.
The sexual cycle in domestic cats has been exploited to develop experimental genetics in T. gondii by crossing different strains and developing genetic linkage maps based on the segregation of genetic markers among haploid progeny [ 20].
The FAE1 and self- in compatibility geneself- in compatibility respective linkagenesoups based on the segregation of the twerearkers in the F2 population of Y514 × Y517.
Transgenic plants for lines 1, 2 and 9 (T1 generation) were selfed for two generations and homozygous T3 lines were identified based on the segregation of the kanamycin resistance marker.
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