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The resolution based on marker gene locations is high but the numbers of markers used in previous studies were limited.
Therefore, the prediction of the functional capabilities of a microbial community based on marker gene data would be highly beneficial.
This problem was addressed by constructing expression vectors based on marker gene expression driven by the weak ARG4 promoter (Pichia Pool, Fig. 2).
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Thus, it is quite perspicuous that MIP harbors different biochemical traits from the organisms, which appear to be its close relatives on the basis of phylogenetic analysis based on marker genes (Table 1).
In comparison to assignments based on marker genes, it has the advantages that it makes assignments across all domains of life and that corresponding abundance estimates from shotgun sequences are less affected by copy number variations of individual genes.
This indicates that phylogenetic classification of genus Brucella based on marker genes, for instance multi locus sequence typing [ 22], show a surprising similarity to the organism's total gene content.
Since the phylogeny recreated by the pan-genomic analyses resemble the pair-wise BLAST comparisons, it appears that gene similarity in the species of genus Brucella is concordant to current taxonomy based on marker genes and similar methods [ 9].
Phylogenetic profiles describe each genome in terms of the presence and absence of proteins from different homologous or orthologous gene sets: early genome trees based on this type of representation were largely congruent with reference trees based on marker genes, which was taken as evidence that genomic data could be used to infer higher-order relationships among groups of organisms [ 10, 11].
Because only a small segment of the human chromosome (11p15.5) is required for the viability of AL cells, mutations based on marker genes located in the human chromosome ranging in size up to 140 Mbp of DNA can be detected (Wilson et al. 1999).
This method can efficiently rediscover many entire biological systems, such as histidine biosynthesis genes, given a profile based on any one marker gene for the system.
Based on marker haplotypes, the homozygosity per candidate gene within the LTS genotype collection ranged from 20 to 75%, which significantly exceeded the percentages expected in a single panmictic ryegrass super-population (Table 2).
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