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The CDS overlaps were evaluated again at the genomic sequence level, with each set of evaluation data for the gene annotations being based on manually inspected cDNA evidence using the graphical interface of our Full-parasites and Comparasite databases (also see Additional file 12) [ 29].
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Perhaps the greatest strength of BamView, when embedded in Artemis, is that it allows annotation to be changed based on manually inspecting data from transcriptome sequencing experiments (RNAseq, Otto et al., 2009; Wang et al., 2009).
Quantitative analyses are based on manually selected ground truth data.
Research in the past was based on manually designed screens with minimal software support.
Each identified RGP was manually inspected based on its genomic context and conserved genes located at the flanks of the region.
The resulting polygon-based maps were manually inspected and misclassified polygons were relabeled.
The start sites were manually inspected and altered based on predictions of GLIMMER and GeneHacker.
Gene models overlapping with hits were manually inspected and kept based on subfamily sequence similarity or EST support.
Traditional approaches to proving termination are usually based on inspecting the source code.
Functional groups pertinent to nectary development and nectar production were then manually inspected and grouped based upon TAIR annotations [ 73] and literature searches.
To test this hypothesis, we used the program tandem repeats finder [ 35], tabulated all repeat arrays on the 11 mega base chromosomes (data not shown) and then manually inspected repeats with a size >10 bp and a copy number >10 for aligned reads.
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