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Reads per gene were counted based on manually corrected gene structures stored in gff3 format and the sorted SAM files by htseq-count (option t = CDS) that is part of HTSeq version 0.5.4 (http://www-huber.embl.de/users/anders/HTSeq/doc/overview.html).html
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Quantitative analyses are based on manually selected ground truth data.
Research in the past was based on manually designed screens with minimal software support.
Each algorithm overestimated MBF compared to MBF based on manually defined blood pool TACs.
Firstly we have shown that the AutoEPG algorithm is capable of analyzing large volumes of data (∼2500 single pumps were analysed in the case of one strain) with high levels of accuracy, based on the comparison of manually corrected data.
Ambiguous and incorrectly called bases were manually corrected using Chromas Lite software, version 2.01 (Technelysium Pty Ltd).
Based on the corrected data, we can conduct association tests correcting for population stratification.
Analyses were based on the corrected concentrations.
Putative PLD gene models were further analysed based on characteristic conserved motifs and manually corrected when needed.
The alignment around the flanking region of the PR-domain was manually corrected based on the exon-intron structure.
Frameshift errors due to 454 homopolymer sequencing errors were identified and manually corrected based on the gapped BLASTX alignment to the closest protein sequences from NR.
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